Journal: Journal of molecular and genetic medicine : an international journal of biomedical research

Article Title: Differences in influenza virus receptors in chickens and ducks: Implications for interspecies transmission

doi:

Figure Lengend Snippet: Widespread presence of SAα2,6 Gal (SNA lectin) and SAα2,3 Gal (MAA II lectin) receptors in many of organs of chickens (A) and ducks (B) . Composite confocal images show distribution of SAα2,6 Gal receptors (green) and SAα2,3 Gal receptors (red) with nuclear staining (blue). 1. bronchial epithelium, 2. bronchiolar epithelium, 3. alveolar lining, 4. muscle fibre, 5. nucleus, 6. myocardium, 7. connective tissue, 8. endocardium, 9. convoluted tubule 10. vascular endothelium,, 11. neuronal tissue, 12. meninges.

Article Snippet: Sections were pre-soaked in TBS and blocked using a biotin-streptavidin blocking kit (Vector Laboratories) according to manufacturer's instructions, followed by 4°C overnight incubation with fluorescein isothiocyanate (FITC) labelled SNA or FITC labelled MAA I, and biotinylated MAA II lectin each at a concentration of 10μg/ml.

Techniques: Staining

Journal: Journal of molecular and genetic medicine : an international journal of biomedical research

Article Title: Differences in influenza virus receptors in chickens and ducks: Implications for interspecies transmission

doi:

Figure Lengend Snippet: Differential expression of SAα(2,3)Gal receptor subtypes in chicken and duck tracheae and large intestines. MAA I (SAα(2,3)-Gal β(1-4) Glc NAc detection; green) and MAA II (SAα(2,3)-Gal β(1-3) Gal NAc detection; red) were used to distinguish SAα(2,3)-Gal receptor subtypes at the third sugar residue position. (A) Chicken trachea expresses SAα(2,3)-Gal β(1-4) Glc NAc receptor (MAA I lectin) more strongly along the epithelial lining than SAα(2,3) Gal β(1-3) Gal NAc (MAA II lectin). Both SAα(2,3)-Gal receptor subtypes are clearly present in the sub-epithelial region (see also MAA II lectin staining in B). In duck trachea, by contrast, minimal SA α(2,3)-Gal β(1-4) Glc NAc receptor subtype (MAA I lectin) is detected along the epithelium. Only the mucosal glands are MAA I positive. In duck trachea, SA α(2,3)-Gal β(1-3) Gal NAc receptor (MAA II lectin) is the main subtype with distribution along the epithelial lining and in the mucosa (see also MAA II lectin staining in B). (B) Chicken large intestine expresses similar levels of both SAα(2,3)-Gal β1-4 Gal NAc receptor (MAA I lectin) and SAα(2,3)-Gal β(1-3) Gal NAc (MAA II lectin) along the epithelial lining. In duck large intestine, SA α(2,3)-Gal β(1-3) Gal NAc receptor (MAA II lectin) is the main subtype with distribution along the epithelial lining and in the mucosa. Similar observations were made in small intestines of chicken and duck (data not shown). 1. epithelial lining 2. intra-epithelial mucous gland 3. epithelial lining of the villus 4. goblet cell.

Article Snippet: Sections were pre-soaked in TBS and blocked using a biotin-streptavidin blocking kit (Vector Laboratories) according to manufacturer's instructions, followed by 4°C overnight incubation with fluorescein isothiocyanate (FITC) labelled SNA or FITC labelled MAA I, and biotinylated MAA II lectin each at a concentration of 10μg/ml.

Techniques: Expressing, Staining

Journal: Neuropharmacology

Article Title: Neural substrates for the distinct effects of presynaptic group III metabotropic glutamate receptors on extinction of contextual fear conditioning in mice

doi: 10.1016/j.neuropharm.2012.05.025

Figure Lengend Snippet: List of the concentrations and combinations of primary and secondary antibodies.

Article Snippet: Sections were first incubated in blocking buffer composed of 2% NGS in PBS for 1 h and then overnight at RT with 5 μg/ml biotinylated anti-PHA-L (Vector) in PBS containing 0.3% TX and 1% NGS.

Techniques: Sequencing, Plasmid Preparation